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In the description fields of basisOfRecord, the links to the different categories is not up to date and need to match the revision. E.g. in Table 4: Recommended fields for Occurrence core for ddPCR/qP…
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Hi @benjjneb,
I am having issues with dada2's assignTaxonomy output. Performing the dada2 workflow as usual for metabarcoding analysis, I have inferred ~8000 ASVs from COI amplicon sequence reads (…
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Hi There!
I'm having issues on the step where you use cutadapt to remove the primers. I am using R in RStudio in Windows 10. I keep getting the following error after running the code below.
"cu…
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The person or people making the observation is a critical piece of information. It should be added to this sentence
> While core files store ubiquitous data on the '**who**, what, where and when' …
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Dear all,
I sequenced the v3v4 region of 16s. I have some sequences which are shorter than 400 bp after merging. Would you recommend to remove those short sequences and at which length would you ma…
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1) Just a reminder that we agreed that the categories in the decision tree should be click-able so the reader could easily jump to the relevant explanation/examples/standards of the category
2) May…
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in section 1.3: "Without careful calibration, biological observations derived from eDNA should be thought of "observed presences" or perhaps "relative abundances" (where the relationship is to other o…
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Hey guys,
first of all, thanks for the great work setting up and - most importantly - documenting this pipeline.
I am a newby in the field of bioinformatics and trying to create my first COI cu…
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I do not understand the objective of this paragraph, it mixes several things and I see different problems.
1/
"eDNA is thus a sample type, not a method, including DNA derived from any environmen…
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![PLNmodels](https://user-images.githubusercontent.com/57043568/97624874-b6d49880-19f5-11eb-8b93-9d05fc620f87.png)
![error](https://user-images.githubusercontent.com/57043568/97624882-bb00b600-19f5…