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Hello,
I'm using ITSxpress in combination with Nanopore sequencing reads, and find that only 4-6% of the sequenced reads are annotated as ITS reads.
We are having some issues with the PCR, so i…
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I am running openPrimeR on a set of 65 sequences to develop primers for amplicon sequencing. I previously had the package up and running properly on a dummy dataset, but now that I'm running on my act…
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Hi Drs,
I tried to use this software to run learnErrors. I have been running for three or four days with no results, how can I solve it?
reads.in reads.out
Gr1m1-1_R1_00…
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Hi there, I have a problem about processing time series data using dada2 (groundwater). I took over a project that has been running already for 9 years and has been ongoing. My former colleague ran pr…
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Hi @benjjneb,
I've been processing some old amplicon sequencing sets with dada2 recently with no trouble (I'm a big fan) . However the last set of paired reads seems to have a mix of primer orienta…
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Hi, am running 16s Hifi demultiplexed analysis, after running the last line of code in this chunk:
meta_ana
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Hello,
I have one Miseq run (2 x 300 bp) that I spent all the sequencing effort in just one sample (V3-V4_16S amplicon sequencing). After removing the primers using cutadapt, the amount of the data…
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Hello everyone,
I am analyzing 16S amplicon sequencing with DADA2 and using phyloseq to get the bacterial composition in the community. I try to import the metadata table in R so that I can later u…
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Hi developers,
Recently, I used seqkit amplicon to extract the target sequence from the compressed FASTQ file by giving primer sequence. I downloaded the latest version (v2.7.0) and the previous ve…
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Using qiime2-amplicon-2024.2 with CLI
qc-rarefy errored on the first sample that is in the unrarefied and not in the rarefied dataset
Code:
```
qiime gemelli rpca \
--i-table ../data/core_d…