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Hi, I used https://doi.plutof.ut.ee/doi/10.15156/BIO/2959334 for ITS taxonomic assignment. I run taxa
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Hello there
Thanks a lot already for the work on this package!
I am trying to cluster 34,937,058 sequences of about 1000bp (18S amplicons) contained in a single fasta file, I'm using the following…
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See this as a workflow:
```
Building OTUs, would require that your reads align very nicely. That is pretty hard with Nanopore reads, and you end up with a lot of singletons. I have tried clusterin…
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Hi,
I have been interested in using your pipeline with ONT long-read data with UMIs that are generated by colleagues in my lab.
To begin with, I tried to execute the pipeline with the example data…
camcl updated
2 weeks ago
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**Describe the bug**
When running the following cmd, `CRISPRessoAggregate --prefix tmp/CRISPResso_on_BNL17517_additional_80k --prefix tmp/CRISPResso_on_BNL17517_additional_80k-160k --name "80k"` erro…
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### Ask away!
I am currently trying to use minimap on a ONT dataset to get a finer resolution, ideally down to species, on some full length amplicons.
When I run this dataset using kraken as the cl…
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I am using a custom amplicon panel for wastewater sequencing and would like to know the process of adding this to the primer sets folder.
E.g. The naming conventions needed and how to go about gene…
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Hello,
We are using CRISPResso2 pooled to map reads to multiple amplicons and it's working very nicely as long as the guide matches the amplicon perfectly. However, we also have cases where a guid…
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Dear Sirs, I have some problem about tmp file which was not created. I ran the job in linux server. There is some error in below:
passedQC_iF02_iR09.fasta contains 17 reads.
--> Low number of read…
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In the fct find_pcr_products, the products that lies outside the minimum and maximum product size is filtered out.
This could potentially remove relevant PCR products that lies just outside the boun…