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For analyzing ITS amplicon data, in filterAndTrim function "out
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Hi,
I'm using an amplicon kit which has tiled amplicons. These can create super amplicons, where the forward primer for amplicon 1 can form an 'super' amplicon with the reverse primer of amplicon2.
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### Ask away!
I am running the wf-amplicon on PCR amplicons covering a complete virus genome (4 overlapping long amplicons per sample, pooled in one barcode) and map the reads to a reference genome o…
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Dear CAMISIM team,
I am starting to try and use CAMISIM for a bunch of projects we have, but wonder if all of them will fit. For instance, is it possible to use CAMISIM for simulating amplicon sequ…
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@k-florek @fanninpm @erinyoung @tives82 @DrB-S
I have been using Cecret for all things SARS-CoV-2 and our research lab just started to work up a NGS methodology for Human Norovirus sequen…
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Hi Felix,
I'm hoping you can help with an issue I'm having with paired end sequencing. I'm sequencing an amplicon that is 350bp long. The first ~300 bases on the forward read match what I expect. Bio…
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The current technique that the assembled version is using to detect amplicons has two problems:
1. Some lengths are identified as amplicons when they shouldn't be.
2. Some lengths are not identifi…
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Hello! Thanks for putting together CliqueSNV - the ability to leverage long reads should be really powerful across different fields I think. I was curious if it would be possible to use long reads tha…
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Hello
Do you have plans to make amplicon_sorter available to install by Conda? It would be great if it was available as a package with all dependencies, i am having some trouble to install some of t…
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Dear all,
I did amplicon sequencing (16S) from woodchip bioreactors that were used to clean water contaminated with pesticides. I am very new to the field and I am still learning how to analyze the d…