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Hi,
I need help with two problems I'm having with your tool on my M1 Mac. Here's what's going on:
Problem with Minimap2: I intended to use Minimap2. When i start the tool using minimap2, it gave…
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Dear developers,
This tools looks super interesting! I wonder if you have tried it coupled with Read Until functionality for cDNA or other "short" long reads (e.g. 1-2kbp).
If not, do you think …
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Hello,
I can build index for Mus_musculus.GRCm38.dna_sm.toplevel.fa, but when build for Mus_musculus.GRCm38.cdna.all.fa, there is a bug:
"rsem-extract-reference-transcripts Mus_musculus.GRCm38.c…
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url where this behavior shows up:
0. https://www.artstation.com/projects/Ya9LZ3.json
1. https://www.artstation.com/artwork/Ya9LZ3
2. https://cdna.artstation.com/p/assets/images/images/023/424/12…
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## patch-seq assay
Label: patch-seq assay
Alternative term(s): patch clamp and RNA sequencing assay
Definition: A single-cell RNA sequencing assay that takes a patch clamp recording from a cell a…
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Hello,
I'd like to request a New Term: "oligo-dT primed Total RNA".
* Editor preferred term: "oligo-dT primed Total RNA".
* Alternative terms: oligo-dT primed cDNA -or- cDNA arising from oligo-…
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Hi,
During some comparisons of STAR and kallisto we saw that the number of genes/transcripts that STAR aligns to and outputs is much larger than kallisto. This obviously makes sense and is expected…
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I have tried to align the [cDNA reads]( http://s3.amazonaws.com/nanopore-human-wgs/rna/fastq/NA12878-cDNA_All_Guppy_3.2.6.fastq.gz) using minimap2 but only a small number of reads align to the human g…
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we sequencing many ONT cDNA RNA data from different tissues, Can I use this software to analyze this data? Thanks
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code:
cut -f 1 -d ' ' ./SQANTI3_1_output/muscle_corrected.fasta > ./SQANTI3_1_output/cdna.new.fa
grep '^>' ./SQANTI3_1_output/cdna.new.fa | cut -d ' ' -f 1 | sed 's/^>//g' > /home/D…