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https://github.com/torognes/vsearch/blob/e79adfd5c77e4a18c218b5aafa430344127e4a9f/src/vsearch.cc#L4744
what happens to reads with a q20 score when i set fastq_max to 90?
using this command
v…
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I am trying to correct the reads (~190x coverage, 1.2Gb genome), but I have been having some issues with the "correct" mode in Dorado.
I ran dorado-0.8.2-win64 using the "dorado correct porechopp…
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Hi, I have encountered this error both using the Docker container provided as well as a local installation. I created a tb-profiler report with default arguments from FASTQ with `tb-profiler profile -…
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This is the new Illumina competitor from Element Biosciences @Elembio
People are using it amplicons: [qiime2 forums xref](https://forum.qiime2.org/t/dada2-low-non-chimeric-input-after-denoising/317…
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I would like to see instructions for analysing CITE-seq data. For example, my data looks like
```
$ ls *
CITE:
BH_pool1_S1_L001_I1_001.fastq.gz BH_pool1_S1_L002_R1_001.fastq.gz BH_pool1_S1_L00…
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Dear Doctor
Today, my TRUST4 suddenly had a small problem:/ run-trust4 -f hg38_bcrtcr.fa --ref human_IMGT+C.fa
-1 /data/zhanqh/data_all/SRR_data/SRR29767032/SRR29767032_2.fastq.gz
-2 /data/zhanqh/…
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cutadapters.wdl crashes if it encounters and empty fastq.gz file, crashing the whole pipeline. An exception must be added to deal with empty fastq files.
Example of an error message below:
```
…
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Hi,
When I run seqkit rmdup "seqkit rmdup -j 20 -s my.fastq > my_rmdup.fastq"
The Seqkit got killed and no other error message is ouput. My fastq file is 35G, and I have 126G Mem in the server. …
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Hi Felix,
I'm hoping you can help with an issue I'm having with paired end sequencing. I'm sequencing an amplicon that is 350bp long. The first ~300 bases on the forward read match what I expect. Bio…
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I have forward and reverse FASTQ files from Illumina paired-end sequencing, and I'm merging these reads using `vsearch`, which works great. However, I need to filter the reads based on a quality score…