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https://mp.weixin.qq.com/s/5kwfiapfBKVSQmo5Zuh6EA
ixxmu updated
3 weeks ago
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Hello,
thanks for develop these tools.
I just have 1 questions:
how to extract full-length L1 from the GTF you build (https://labshare.cshl.edu/shares/mhammelllab/www-data/TEtranscripts/TE_G…
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https://mp.weixin.qq.com/s/cGJrOF6SCGNg67a91CD3Iw
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The following code does not find a `cas9` hit, when it should. Changing the input guide to uppercase solves the issue
```
from pydna.dseqrecord import Dseqrecord
from pydna.crispr import cas9, pr…
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https://mp.weixin.qq.com/s/jldxPNo_ofeNlfx26TqnoQ
ixxmu updated
3 months ago
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Hello!
I am interested in using ScreenPro2/ScreenPro to analyze reads from a dual-guide CRISPR screen. Specifically, I wanted to get counts from my reads files and map them to my dual-guide library…
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* crispector version: 1.0.4
* Python version: 3.7
* Operating System: Ubuntu (64-bit)
### Description
I would like to compare WES data from two cell lines (parental and knock-out). Knock out cel…
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Hello!
Running:
```r
library(depmap)
```
gives me the following error:
```
Error: package or namespace load failed for ‘depmap’:
.onLoad failed in loadNamespace() for 'depmap', detail…
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https://mp.weixin.qq.com/s/jo_B6rZW4ygvo4j5t0Cg8w
ixxmu updated
2 weeks ago
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I ran CRISPRESSO2 using on a CRISPR/Cas9 edited loci, while the "Allele plots" shows several INDELs, the "Allele_frequency_table" does not reflect the same INDELs and recognize the INDELs as "TRUE" fo…