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Hi,
Thanks for the work on this tool! I'm helping a collaborator design guide RNAs for a CRISPR screen in mouse, and so far it's been really useful. However, I'm having an issue when trying to desi…
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Efficacy of replicate correlation across guises could be inflated based on biased selection
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Hi,
I want to use this tool to design sgRNA for my study plant genome, but I don't know how to use it when git clone in my Linux. So, can you give me a simple tutorial?
Thanks.
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Hi,
I see the following error:
Error in data.frame(sgRNA = quant_ret$sgRNA, sequence = quant_ret$sequence) :
arguments imply differing number of rows: 79633, 79637
> traceback()
3: stop(ge…
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Hi
I have three questions about features I cannot see in the documentation but wonder if they exist:
1) When a coding sequence is provided the Frameshift_analysis.txt gives a summary of the numb…
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Which one is a better representation of the process:
We used to do this:
but now I think this could be more accurate:
It just need the interaction node Process (https://github.com/Syn…
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add option to return the ggplot object to user
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The maximum mismatch parameter for `-n/--seedmms` is 3 but in fact, Bowtie will display hits for a sequence containing a maximum of 2 mismatches in the seed. I discovered this in v0.12.7 but it is act…
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Hi Dylan,
I'm here to inform you there is a typo in the `sgRNA_design()`. On line 156, it reads `Efficency_score`, but this should read `Efficiency_score`. There were some other minor quirks that I…
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Unfortunately, when I try and follow the tutorial available from CRAN, the function `run_sgrna_quant` was not found. Could you resolve this issue?