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Both [Inspector](https://genomebiology.biomedcentral.com/articles/10.1186/s13059-021-02527-4) and [Merqury](https://genomebiology.biomedcentral.com/articles/10.1186/s13059-020-02134-9) produce QV valu…
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Hi Hannes,
I would like to ask for your advice regarding a problem that I have with the k-mer histogram of sturgeon species probably allotetraploid (Acipenser naccarii).
The problem is that the h…
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I am curious as to the way Blobtools2 calculates N50? For the exact same dataset I get an N50 of 706kb with Quast and an N50 of 710kb from Blobtools2 (via snailplot). I am not overly concerned with a …
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Hi @douglowe
Currently the pipeline uses a singularity image for the blobtools rule, which can be a problem for users on different systems.
Blobtool installation infor here https://blobtoolkit…
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**I am wondering why majority of my sequences have no-hits** , I used blastn
One extra question please, **(How) Can I filter based on phylum name?** so just choose a phylum name to be filtered …
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Hello, I've tried this on the command line though the conda install, and through docker. I'm getting a UserWarning: Contig names in the Busco file did not match dataset identifiers. But the contig nam…
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Write a [pysam](https://github.com/pysam-developers/pysam) nf-core module.
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hey
I am just working through the code [here](https://blobtools.readme.io/docs/taxonomy-file)
```sh
# Download database
wget ftp://ftp.uniprot.org/pub/databases/uniprot/current_release/knowled…
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Hi @simoncchu
I was able to run the Preprocess and Collect steps, but during assembly this is the error message that I get:
```
First round assembly and merger...
**
***
*
Stage 1: 0%
Sta…
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Hello,
This is similar to [issue#69](https://github.com/chhylp123/hifiasm/issues/69) but for me it is unsolved yet. I am running hifiasm v 0.14.2-r315 to assemble ~3.5Gb genome. My hifi fastq files…