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Hi,
I found that there are many incomplete gene models in mikado output gff file, is there a way to update these models to complete gene models?
Best,
Kun
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https://mp.weixin.qq.com/s/y9iZ-leL8MrVg_nQEnO-Eg
ixxmu updated
2 years ago
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https://mp.weixin.qq.com/s/y9iZ-leL8MrVg_nQEnO-Eg
ixxmu updated
2 years ago
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## Preferred term label
Iso-seq or ScISOr-seq
## Synonyms
PacBio Iso-seq
## Textual definition
https://www.pacb.com/blog/introduction-of-the-iso-seq-method-state-of-the-art-for-full-…
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I found there is one description as the following
" ....
I work mostly with [**PacBio transcriptome (Iso-Seq data**)](https://github.com/PacificBiosciences/cDNA_primer/wiki), these are all used for…
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**User story**
As a traction user, I would like to track new fields to assist with sample sheet generation.
> Sequencing of Microbial, HiFi and ULI libraries. If binding kit v2.2 is used we need…
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I have long read nanopore reads that I have collapsed into a gtf transcriptome via FLAIR align/correct/collapse, to be used as input for SQANTI3 (version 4.1). Furthermore, using FLAIR quantify, I get…
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Hello,
I did run SQANTI3 using the below command line.
sqanti3_qc.py collapsed.gtf gencode.v30.annotation.gtf hg38.fa --cage_peak hg38.cage_peak_phase1and2combined_coord.bed --polyA_motif_list m…
tay45 updated
2 years ago
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Hi,
I am running the Iso-seq-SQANTI3 pipeline on multiple samples, my data is sequenced in both Pacbio long read sequencing and Illumina short read sequencing. I still don't understand how to include…
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Hi,
I am using the last version of SQANTI 3 and I have a problem with the evidence transcript / Gene association when the overlap is very limited or only on UTR parts. In my genomes (small fungal ge…