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Hello, I am an avid user of the rMATS tool and have found many splicing sites and events. Now I have obtained alternative spling events of the differences between the two groups of data. For example, …
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Hello there,
Today I started to learn how to use this great package, this is going to be very useful for at least two publications I am trying to wrap up :)
I am interested to use these schemat…
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### Description of feature
Differential splicing is a frequent consequence observed in RNA-Seq data. rMATS (https://rnaseq-mats.sourceforge.net/) is the most frequently used analysis tool, providing …
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I really love this package and have been working on a little shiny app so users can look at alternative splicing in different cell types. However, it looks like the app has an interesting way of dete…
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The [rMATS](https://rnaseq-mats.sourceforge.net) tool relies on the Bioconductor package [PAIRADISE](https://bioconductor.org/packages/release/bioc/html/PAIRADISE.html) in order run the paired statist…
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Hi,
I have followed the ASpli tutorial from: https://rdrr.io/bioc/ASpli/src/inst/doc/ASpli.R and ran the commands (the ones that weren't commented out), although, when I try to visualize the altern…
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I've seen a few issues posted here that describe lots of alternative first and alternative last events. These could simply be due to a noisy reference gtf. SUPPA makes it easy to limit this issue by d…
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Top to bottom workflow with all the steps for the projects
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Hey guys, thanks for the tool! Very useful!
I'm a bit confused about the "num_reps" parameter. And also who it relates to the event probabilities.
I have implemented an alternative splicing (AS)…
ls233 updated
9 months ago
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Hello, I am following the pipeline of finding alternative splicing analysis using QoRTs and JunctionSeq.
I am working on tomato rna-seq data
I have used HISAT2 for alignment and used "--minMAPQ 60".…