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https://mp.weixin.qq.com/s/Ydm6X7AUwr5WYM7dCZtmzA
ixxmu updated
1 month ago
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Having issues creating a plotly plot for a specific dataset and parameters using iPad. See attached screenshots.
![IMG_1254](https://github.com/user-attachments/assets/1f3e4657-1f3b-4112-a0c1-50fea…
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Hi @GregorySchwartz, I am trying to run `too-many-cells` on a linux cluster where I don't have sudo access and docker is not a viable installation options. Can I use prebuilt linux binaries of `too-ma…
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Hello,
I'm trying to run velocyto using 10x data and GTFs for macaca mulatta from NCBI. The command is:
velocyto run -o ./outs -b barcodes.tsv.gz --samtools-threads 11 -m mm10_rmsk.rename.gtf 43…
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Dear Tinyi,
Thanks so much for developing (and maintaining) such great software.
This is more of a question rather than an issue. We were wondering whether it would be possible to start from know…
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I have an scRNA-seq dataset of patient-derived fibroblast cell cultures. The code does not handle the case of just one cell type.
```r
fastruvIII.nb(delayedCounts, matrix(1, nrow = ncol(delayedCou…
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Some questions
* Will FACS sorting disturb RNA?
* How should we preserve the cells post-sorting and pre-RNA isolation?
* How should we isolate RNA?
* What markers should we sort on?
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Hello,
In the html tutorial [here](https://carmonalab.github.io/GeneNMF.demo/NMF_demo_PBMC.html#consistent-nmf-meta-programs-across-multiple-samples) the assay used is sct which is sc transformed dat…
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I was hoping to use CellOT on full scRNA-seq data and was wondering what the training times for that should look like and if there is any way to accelerate training. I'm currently running scGen to get…
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https://mp.weixin.qq.com/s/ReaV52HlFpHY9gLvV019qw
ixxmu updated
1 month ago