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Hi, I'm trying to do _de novo_ assembly of a unicellular eukaryote genome that has an average size of 114 Mbp. I've successfully run Masurca 3.3.5 with pacbio `+` Illumina pair reads. Afterward, I wan…
zeyak updated
4 years ago
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Estimated genome size text file is showing the value that is half the genome size. Should I be concerned?
nm100 updated
6 years ago
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Hi,
I am running MaSurCa version 3.2.6 but I got numerous Broken pipe massages which probably lead that it failed.
Here is my config file:
```
# example configuration file
# DATA is specifi…
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ONE:
polca.sh -a tangyu.racon.v2.fasta -r 'wHADPI077755-37_1.fq.gz'
[Wed Dec 18 23:43:13 CST 2019] Creating BWA index for tangyu.racon.v2.fasta
TWO as REDME said:
polca.sh -a tangyu.racon.v2.fa…
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Hi, I'm actually using MaSuRCA-3.2.6 to assemble my genome and a ran the fallowing script:
```
#PBS -S /bin/bash
#PBS -l nodes=1:ppn=8:bigmem,mem=100gb
#PBS -e /pandata/ACG-0006_002…
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Hi,
I am trying to use the chromosome scaffolder tool in MaSurCa.
I dont understand why I keep getting the error "Wrong type/extension for the file, must be .fa, .fasta or .fastq"
When the refe…
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Dear Aleksey,
I have run the latest version on the grid (using slurm) to create the megarreads. After the create_megarreads job array finished I simply rerun ./assemble.sh and I obtained a flye ass…
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we use masurca the assembly the genome, we got the ESTIMATED_GENOME_SIZE.txt file, the value in ESTIMATED_GENOME_SIZE.txt were twice the size of the genome size
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Hello,
I am trying to assemble a mollusc genome (1.35 Gb) using Masurca with Illumina and PacBio reads.
I manage to get the mr_pass2 folder with all the job on the grid successful.
After this ste…
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I've been running an assembly for quite a while using MaSuRCA 3.2.8 and we may have to shut the machine down. The assembly is currently in the 1-overlapper step and I was just wanting to check if we c…