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**Hello, I currently want to reproduce the results in SEVtras using data from 15 normal tissues. When I was running the SEVtras.ESAI_calculator function, the following error occurred. I tried for a lo…
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https://mp.weixin.qq.com/s/G2mD2sOdyYqixOVtM5ZWUQ
ixxmu updated
3 weeks ago
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Hello,
I would like to know what the minimum requirement in sample size is for bulk RNAseq in order to trust the inferred cell fraction. Do we need at least 10, 20 samples ?
Thanks !
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Hello,
Thank you for developing this nice tool.
I have single-cell RNA sequencing (scRNA-seq) data from tissues generated by crossing two distinct mouse strains. I am aware of the single nucleotide …
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Exon annotations cause conflict in velocyto, presumably related to issue #73 there: https://github.com/velocyto-team/velocyto.py/issues/73
```
% velocyto run10x sample1 human_GRCh38_optimized_v1…
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Hi, all
When I use monocle3 default parameters to `plot_cell_trajectory` , I got a not fully connected graph, as below:
![image](https://user-images.githubusercontent.com/32219336/53737415-70006980…
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@sqjin
I am working on CellChat-vignette in your tutorial.
when I run `cellchat
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We try to use ecotyper in our scRNA-Seq datasets with de novo model in ecotyper.
We need the Token of CIBERSORTx for docker images. And we have asked for the Token from the website.
Could you share …
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Hi,
Thanks for creating such a useful tool. I have managed to successfully install `inactiveXX` with all dependencies. I am not able to get it work. I appreciate if you can help me in resolving t…
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Hello,
I generated a UMAP trajectory in Monocle3 and am able to plot any genes of interest across pseudotime no problem with plot_cell_trajectory. However, we would like to be able to discover new …