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As we run the assign taxanomy and add species command. but the result contain only NA in species column
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I am running openPrimeR on a set of 65 sequences to develop primers for amplicon sequencing. I previously had the package up and running properly on a dummy dataset, but now that I'm running on my act…
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Hi, I am trying to detect ecDNAs from my circle-seq data,is AA suitable for that?
and I am going to use prepareAA and CNVkit to get the CNV bed files which will be feeded to AA.
If not, could you gi…
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Hello!
I tried now several times running "learnErrors" of DADA2 in R. Loading the package worked fine and every step described until that. It runs about an hour, gives as output "**5485896480 total…
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Hello!
After the remove chimeras step in DADA2 v1.2, I'm getting the following errors in my log output. Any help would be greatly appreciated!
```
abundance forward reverse nmatch nmismat…
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I assume these are similar to the missing files: [here](https://github.com/artic-network/primer-schemes/tree/master/nCoV-2019/V4.1).
I just added the following headers:
chrom, Start, End, Amplicon_I…
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Dear nanopore devs,
I'm having issues getting mini_assemble to run on human (HG001/NA12978) data. I've successfully ran it on smaller assemblies before, but a ~30x human genome seems to be causing …
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The goal of the guard rails is to provide users with informative warnings when their analysis could potentially be compromised. We want to create a system where we can have multiple guard rails for di…
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Hi there, I have a problem about processing time series data using dada2 (groundwater). I took over a project that has been running already for 9 years and has been ongoing. My former colleague ran pr…
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hello,
I am working on 16s amplicon sequencing and I wanted to create a SILVA reference database using this tutorial:
[https://constax.readthedocs.io/en/latest/tutorial3.html](url)
so I created a s…