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I have downloaded a publicly available data set and I have created a Seurat object.
I need to pull the raw count data for analysis in another package. But when I run:
head(obj@assays[["RNA"]]@c…
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Hello,
I ran the following command:
```
appendCB/src/appendCB --append AAACCCACACACACTA Aligned.sortedByCoord.out.bam AAACCCACACACACTA.tagged.bam
```
but the output bam `AAACCCACACACACTA.tagged…
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https://mp.weixin.qq.com/s/6ZuGqbBuEHkQ7M-dmfY-wg
ixxmu updated
2 years ago
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Hi,
Thank you very much for putting together this article and GitHub content!
The journal didn't have a comment section, and I think some authors believe PubPeer has a negative connotation.
I…
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Hi,
I have 8 10X multiome (scRNA+ATAC) samples (2 groups: stimulated; unstimulated). The analysis is based on the Seurat and Signac multiome and integration vignettes.
After cell annotation, I perf…
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Dear developers,
I could successfully create a inferCNV object from my 10X scRNA-Seq datasets. However, when I then try to create the object for the interactive analysis with the NGCHM-tool I'm getti…
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I encountered the problem when I used your data and performed your original codes.
[scRNA-seq Analysis (Practice).md](https://github.com/theislab/single-cell-tutorial/files/6416362/scRNA-seq.Analysi…
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Perform all of the same plots using the filtered data as we had done with the unfiltered data and answer the following questions:
* Report the number of cells left for each sample.
* Did we lose a…
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**Work in progress**
While working on single-cell sequencing terms, I realised that the 'transcription profiling by high throughput sequencing' branch should be reviewed:
- [x] ‘DNA-seq’ and 'wh…
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https://mp.weixin.qq.com/s/W9bfwCwhqKxGJ1Aw_Re24g
ixxmu updated
2 years ago