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Thanks for the great package. Wondering if it can be applied for 5' 10X scRNA-seq data?
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Some of our Sequencing Service providers deliver to us paired-end sequence data in UBAM (unaligned) file format containing both forward and reverse reads. Do you think it would be helpful to many us…
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May i ask 1 question:
if data is already pseudobulk object from scRNAseq data with logCPM value, how can i change it back to a seurat object with counts value? Can i still use above method to turn …
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May i ask 1 question:
if data is already pseudobulk object from scRNAseq data with logCPM value, how can i change it back to a seurat object with counts value? Can i still use above method to turn …
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May i ask 1 question:
if data is already pseudobulk object from scRNAseq data with logCPM value, how can i change it back to a seurat object with counts value? Can i still use above method to turn …
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"Whole-genome sequencing and targeted amplicon capture" says:
> "Do not mark duplicates in the BAM files for samples sequenced by this method"
However, in the BAM file preparation, it is writt…
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I had recently did a Nanopore sequencing run for SARS-CoV-2 in MinION with midnight primers 1200 bp amplicons (Nikki Freed protocol) and native barcoding kit (EXP-NBD96). I have earlier used EPI2ME pi…
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@dbacsik says:
> N.b. Also note that some of the samples in this pilot were assigned to the wrong sequencing indices, and this notebook manually corrects this in the results folder. This needs to b…
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As we are evaluating integration methods and testing subsets of datasets, it would be helpful to see the exact breakdown of ScPCA projects and how much of each disease, 10X platform, etc. are included…
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I am trying to analyse RNA-velocity for single-nuclei RNA sequencing data from 10X platform. I am not sure in detail how it calculates the unspliced/spliced rate when I run the command 'velocyto run10…