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Hi there,
congrats on this amazing tool! I was wondering whether you have had the chance of (or planning to) evaluating DADA2 on T-cell/B-cell receptor data. Antigen receptor amplicons are conceptu…
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Hello –
I have a question about combining different sequencing runs, specifically illumina and 454 runs.
For reference - Samples Include:
• Hydrothermal vent marine samples, time series over 10…
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I am applying control-FREEC on custom target gene sequencing data with predesigned custom panels.
I have one dataset with 15991 targeted amplicons distributed on almost all chromosomes. FREEC worked …
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Hi Anita,
I hope you are doing well. I am trying to figure out how HaplotypR works in regards of DNA read reconstruction. Because I am working with markers which length is about 800 bp, I was wonde…
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About amplicon sequencing that uses DADA2 in QIIME2 environment, I wonder is it actually generating ASVs or OTUs? Is that means that as long as I am using DADA2 (no matter where it is embedded) I am a…
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hi,Blatte.
I have a ampliseq data from illumina platform and try to call ITD by getitd, running the code:$python3 $getitd -reference $itdref -anno $itdanno GSH \
$I/Nova373-RDMT09-A144V1-PM2-2019…
moshl updated
4 years ago
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**Hello Ben!
I am running the dada2 of qiime2 on single reads dataset. I work in Linux and get the following error:**
R version 3.5.1 (2018-07-02)
Loading required package: Rcpp
DADA2: 1.10.0…
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Dear Ben,
I wrote you few months ago for guidance on using PacBio for my research. I’m studying the intra-specific diversity of an uncultivated prymnesiophyte alga. I designed specific primers to a…
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Dear DeepSimulator developers,
I would like to use your tool for simulating nanopore reads, resulting from amplicon sequencing. However, all the reads in pass.fastq have exactly the same errors. Are …
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Hello!
I'm trying to process a 16S amplicon sequencing dataset (250 bp, paired end reads, sequenced on a MiSeq2000).
When I try to run LearnErrors though the function just doesn't finish. I'm …