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Hello,
I referred to the method mentioned in https://github.com/BUStools/bustools/issues/49 to construct the barcode whitelist for BD, but the parameters mentioned in that issue `-x 0,0,9,0,21,30,0…
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In the barcode parsing code in bustools correct
https://github.com/BUStools/bustools/blob/master/src/bustools_correct.cpp#L711
it supports barcode files of the format
```
AAAA GGGG TTTT
- TTTT…
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Dear team,
I'm a little bit confused about the build index step. The manual says that it builds a transcriptome index but needs as input a genomic fasta and a gff. I would like to create the count…
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Running bustools 0.39.2 will report the error :
"Error: invalid command inspect
bustools 0.39.2"
Suggest putting into the 'scKB.yaml' :
bustools=0.39.3
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Bustools fails to build on Armel and many other archs, see the complete build log for Armel here: https://buildd.debian.org/status/fetch.php?pkg=bustools&arch=armel&ver=0.40.0-4&stamp=1589233599&raw=0…
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Hi- I'm very interested in trying bustools predict. I have bustools version 0.41.0 and am trying to test various workflows using the 10x v3 PBMC data. I generated files using
`kb count -i GRCh38.rna…
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For several datasets I have pulled from the literature, the median UMIs per barcode predicted by `bustools inspect` is much higher than the actual median UMIs per barcode in the output of `bustools co…
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Dear Laura,
There is a discrepancy between the gene count and transcript count from the count matrices generated by Kallisto. I generated the gene count matrix and tcc matrix from the same set of f…
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Hi I am pretty new to kallisto/bustools so thank you for your help in advance. I followed directions from this issue/Google collab: [Issue #75](https://github.com/BUStools/bustools/issues/75)
I ra…
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I am testing our usual quantification pipelines with `kallisto` and `bustools`, and noticed `bustools capture` seems to have problem with its barcode mode, if the barcodes are long (in this case, 38bp…