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Request: Help with Obtaining STAR Alleles from VCF Files (Illumina Short Read Sequencing, Dragen Variant Caller)
Hi,
I’m looking to get STAR alleles for a set of VCF files generated from Illumina …
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Hi,
I normally work on target capture data from plants and fungi. A colleague of mine is planning to use the Tetrapods-UCE-5Kv1 for birds. He asked me to double-check how much sequencing data to re…
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## Description
- Illumina sequencing runs are not getting cleaned
- The cleaning relies on the timestamp of the sequencing folder (cleaning runs older than certain time)
- The timestamps of the fol…
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I have this samplesheet here that should be fine and passing validation, but I cannot get this to work.
```bash
[Header]
FileFormatVersion,2
RunName,name123456
InstrumentPlatform,NovaSeq
oper…
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Hi CFE-Lab,
We ([VIDRL](https://www.vidrl.org.au/)) are moving from sanger sequencing to HTS sequencing using a custom hybridisation probe panel which will be sequenced on either iSeq or NextSeq. I…
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Hello,
I'm currently working on data preprocessing from multiple Illumina runs.
The first run was sequencend using a v2 kit, while the other runs used v3, which gives me different amplicon sizes (…
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Hello,
Thank you for your great tool!
I have been trying to generate error free illumina reads using the following command:
```
command = [
"mason_simulator",
…
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https://blog.yech.xyz/posts/illumina_sequencing_spec/
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https://gitlab.dev.singlecell.gi.ucsc.edu/ucsc/azul/-/jobs/103847
```
======================================================================
FAIL: test_indexing (integration_test.IndexingIntegrat…
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Per discussion in Twist [here](https://twist.com/a/197793/ch/619193/t/6274868/c/90989657), @evanfields notes that
> We run with adapters specified as -b, meaning could be 5’ or 3’ end, so when an a…