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Hello Vadim and Team,
I am using a spectral library for modified histone peptides that I built in skyline. I have some questions regarding using this library...
1. What is the best way to conver…
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Operating system and version: Windows 11
Mzmine version 4.2.0
I am trying to use the Spectral Library Search function in MZmine 4.2.0. In previous versions (before version 4), I could select and l…
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Things to add and change in the spectral library generation.
A collection of ideas.
- [ ] add entries to existing file
- [ ] Export isotope pattern score
- [ ] option to include/exclude RT or CC…
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Hello,
With the capabilities in the new version, I am interested in using DIA-NN to search for histone PTMs from extracted histones. The since histone tails are highly decorated with PTMs, the main…
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Hello, I have been trying to familiarize myself with the library. However, I encountered a problem.
The spectral library calls SetClientSizeWH. If the call is changed to SetClientSize, the error does…
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Hello, I encountered an issue as described in the title.
Building Spectral Library using DIA-NN v1.9.1
I created a spectral library using DIA-NN 1.9.1 with the following parameters. The output f…
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Hi,
i used a peptide database build by myself to generate a spectral library, however, i found that when i used the library to search, the outcome file is only 1kb with empty information. and i…
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For a user provided spectral library, how does DIA-NN select fragment ions in the library for peptide detection?
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Would it be possible to add a feature that allows users to identify the spectral library uploaded that was created in-house (i.e. the one with the highest accuracy) such that matches made using that l…
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I've been having trouble running my DDA and DIA files in MSFragger using the MSFragger-DIA workflow. The issue seems to be with the mzML DIA files. I've been discussing this on the Fragpipe and openms…