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### Description of the bug
When choosing to include the RSEQC analysis, an error is generated for most of the samples.
Below is an example of the error generated in the work directory for a failed s…
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Hi @EricKutschera , I am currently using rMATs-turbo to detect alternative splicing events between two conditions. From the recently published paper, "A large scale binding and functional map of human…
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Hi,
I want to filter the splicing event by briekit-event-filter funciton. But some error and warnings came out. The GTF and fa files were downloaded from ENSEMBL. I assume that briekit may not suppor…
AIBio updated
2 years ago
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Hi,
I did not see any examples analyzing other splicing types (A5SS, A3SS, MXE, RI) using BRIE. I used brie-event-filter to deal with gff3 of other splicing types, but I can not get gold.gtf or gold.…
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Hello,
I am using briekit-events to extract splicing events from the human gencode v25 annotation file in gff3 format, which has over 55k genes. However, the output file has alternative splicing ev…
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Thanks for your tools! There are 2 sample groups with 3 BAM files per group，but I am counfused with the results, how to filter for differential alternative splicing between the experimental group and …
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I've seen a few issues posted here that describe lots of alternative first and alternative last events. These could simply be due to a noisy reference gtf. SUPPA makes it easy to limit this issue by d…
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Dear Team,
I run Whippet on my paired-end, unstranded, polyA+ RNA-seq dataset. The dataset is of 75bp length (all reads in all samples have this length) and performed in triplicates per condition (…
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Hi Yuanhua,
I want to perform differential splicing of different groups of single cells. Could you provide an example for groups as I did not find this in the manual.
Thanks
ghost updated
2 years ago
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> - Motivation
Alternative splice site selection is inherently competitive and the probability of a given splice site to be used also depends on the strength of neighboring sites. Here, we present a …