SweepFormula: P/x subtraction - Githubissues

AllenInstitute / MIES

Multichannel Igor Electrophysiology Suite

https://alleninstitute.github.io/MIES/user.html

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SweepFormula: P/x subtraction #334

Open timjarsky opened 4 years ago

timjarsky commented 4 years ago

[ ] Split into two issues: One for DAQ and one for display
[ ] Think about doing it in the wavebuilder (check with experts and other software)

Implement p / x subtraction in MIES.

This is a technique used to subtract leak (aka linear) currents from current traces.

Desired workflow:

User builds stimset in waveBuilder e.g. vClamp1
At data acquisition, if user has activated p/x (user defines x; -4 is common), the stimulus set is modified by MIES and then acquired.
In the first sweep, MIES acquires the waveBuilder vClamp1 sweep 1. In the same sweep, immediately following vClamp1, vClamp1 / x is acquired. vClamp1 / x is repeated x times.

So, a stimset that looks like this:

Get acquired like this when x = -4:

When p/4 data is acquired, provide the user with the option of displaying the subtracted traces. The display of subtracted traces could be done using SF.
Subtraction formula: vClamp1 - (x * (average of vclamp / x traces)).

timjarsky commented 1 year ago

Add P/x to DA_Ephys. It will generate a new stimset on-the-fly (as with oodDAQ)
Add P/x sweep formula to do subtraction
Add special P/x epoch info where epochs equal the duration of the user-defined stimset sweep. SF will recognize P/x sweeps using lab notebook data. The sweep formula will average and multiply appropriate epochs together and subtract from the user-defined epoch.