AppliedBioinformatics / runBNG

An easy way to run BioNano genomic analysis
MIT License
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Missing module of pybionano.smapdata #35

Open liangwx332 opened 2 years ago

liangwx332 commented 2 years ago

Hello,

I run runBNG for your example and all dependence has been solved. But I did not get right result, the error as following. Would you give me some suggestions?

Lwx

========================= ============Code=================================== runBNG denovo -t /share/home/biosoft/tools/pipeline/Solve3.7_10192021_74_1/RefAligner/12432.12463rel/ -s /share/home/biosoft/tools/pipeline/Solve3.7_10192021_74_1/ -b Molecules.bnx -T 5 -l 120 -m 8 -j 30 -z 100 -o test

======================================Error=================================== ========================================== De novo assembly starts =============================================

The data generation platform is: saphyr

The bnx file is: /share/home/biosoft/runBNG-master/Examples/Molecules.bnx

The minimum molecule length is (Kb): 120

The minimum label on a molecule is: 8

Maximum backbone intensity is: 0.6

The path to Bionano Solve folder is: /share/home/biosoft/tools/pipeline/Solve3.7_10192021_74_1

The path to Bionano RefAligner folder is: /share/home/biosoft/tools/pipeline/Solve3.7_10192021_74_1/RefAligner/12432.12463rel

The number of threads is: 5

Large jobs maximum memory (GB) is: 128

Small jobs maximum memory (GB) is: 8

The number of threads for each subjob is: 30

The number of iterations is: 5

False Positive Density (/100Kb) [FP]: 2.0

False Negative Rate (%/100) [FN]: 0.10

ScalingSD (Kb^1/2) [sd]: 0.0

SiteSD (Kb) [sf]: 0.12

RelativeSD [sr]: 0.03

The genome size (Mb) is: 100

The xml file is: /share/home/biosoft/tools/pipeline/Solve3.7_10192021_74_1/RefAligner/12432.12463rel/optArguments_nonhaplotype_DLE1_saphyr.xml

The output directory is: test

Traceback (most recent call last): File "/share/home/biosoft/tools/pipeline/Solve3.7_10192021_74_1/Pipeline/1.0/pipelineCL.py", line 11, in import Pipeline File "/share/home/biosoft/tools/pipeline/Solve3.7_10192021_74_1/Pipeline/10192021/Pipeline.py", line 31, in import reports File "/share/home/biosoft/tools/pipeline/Solve3.7_10192021_74_1/Pipeline/10192021/reports.py", line 8, in from varcluster import VariantClustering File "/share/home/biosoft/tools/pipeline/Solve3.7_10192021_74_1/Pipeline/10192021/varcluster.py", line 7, in from pybionano.smapdata import SmapData, SmapFilter ModuleNotFoundError: No module named 'pybionano.smapdata'

liangwx332 commented 2 years ago

I try to search the python module of pybionano.smapdata ,bur without any result. I do not know why does runBNG miss the module?

yuxuanyuan commented 2 years ago

Sorry for the late reply. You may download Bionanao Solve3.5.1 and try again.