CVUA-RRW / FooDMe

A reproducible and scalable snakemake workflow for the analysis of DNA metabarcoding experiments, with a special focus on food and feed samples.
https://cvua-rrw.github.io/FooDMe
BSD 3-Clause "New" or "Revised" License
4 stars 1 forks source link

Add option to deactivate the BLAST default DUST filter for low complexity sequences #67

Closed gregdenay closed 10 months ago

gregdenay commented 10 months ago

t is now possible to desactivate the defualt low-complexity filter of the BLAST search. This can be advantageous if you expect your barcode to contain low-complexity sequences which could prevent getting any match at all. This behaviour can be activated/deactivated by changing the blast_filter_low_complexity from True to False.

The default behaviour (False) uses the default 'DUST' filter of the blast tool: -dust 20 64 1 -soft_masking true.

Checklist