Cardiovascular-Modeling-Laboratory / zlineDetection

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ZlineDetection

Quantify z-line architecture in images of striated muscles.

Reference This Work

Morris, Tessa Altair, et al. "Striated myocyte structural integrity: Automated analysis of sarcomeric z-discs." PLOS Computational Biology 16.3 (2020): e1007676.

Getting Started

  1. Download and open zlineDetection in MATLAB.
  2. To open zlineDetection GUI and initialize analysis, type the following in the MATLAB command line
    zlineDetection
  3. See userGuide.pdf for more information.

Note: If you encounter the error saying "'CONCRT140.dll' required", it's because the run-time component is missing from the system. Install Visual C++ Redistributable Packages for Windows should resolve this issue.

System Requirements

MATLAB Version >= 9.5

Image Processing Toolbox Version 10.3

Statistics and Machine Learning Toolbox Version 11.4

To check the version of MATLAB and which Toolboxes you have installed, type the following in the MATLAB command line:

ver

Versioning

Version Date Update Description
1.2.002 Aug 24, 2020 Fixed bug so that the Excel summary sheet now has the correct number of coverslips per condition
1.2.001 Jan 15, 2020 Added functions to save video frames as separate .tifs and a function that can summarize the video data. The user guide has also been updated with instructions to analyze videos, and descriptions of where the results of measuring the sarcomere length can be found
1.1.001 Jan 15, 2020 Added sarcomere length (distance) calculation for each image. Summary for each coverslip has not been implemented.
1.0.004 Jan 15, 2020 Implemented semantic versioning. Change x-spacing of dots in dot plots. Enhance filenames comparison.
1.0.003 Oct 30, 2019 Fixed bugs so post analysis, multiple runs can be combined.
1.0.002 Oct 27, 2019 Added script to manually remove regions of the background, which were missed by the background substraction.
1.0.001

ZlineDetection uses semantic versioning where the version number follows the convention ... Major Release: new features break backwards compatibility/current features Minor Release: features don’t break any existing features and are compatible with old versions. Patch Release: bug/patch fixes

Authors

License

Acknowledgments