Biobroker

This repository contains the source code for the Biobroker python package. This package contains multiple utilities, but the overall philosophy/goal can be described in a phrase:

Given an input file of any type containing metadata about entities, broker it to any archive and return a human-readable result.

I understand that this is a very ambitious project; for starters, the variety of existing archives is way too high. For now, this package is being developed by extending my knowledge on the european genomics/bio archives hosted by EMBL-EBI. It may be that some archives can't just be adapted, but I will be very happy if this library can be extended to just work with enough archives to ensure a biology laboratory can set up an automatic brokering process that works for them.

Infrastructure summary

The infrastructure, in simple terms, is a very modularized submission system. Each of the components should have a generic python object, that collects all the attributes and expected methods for that class. Then, for each archive, a set of subclasses for each (Or some) of those modules must be defined.

Let's take as an example the "authenticator". Very briefly, authenticator is a module that takes in a password and a username and spits out a token. It also defines the requests, to ensure the token is always provided with the headers.

Each subclass of the GenericAuthenticator defines:

• What is the base_uri of the authentication service
• What is the auth endpoint called to retrieve the token
• What is the process to retrieve the token (Sometimes, like in the WebinApi, requests need to have a specific body)

And so on with all the modules.

All these modules interact with each other, but can be used mostly independently. Some modules are dependent on each other, such as the API module needing the Authenticator in order to send requests. In the end, there is a module that loads all of them, but should be generic enough that only one needs to be defined: the Wrangler.

A graphical representation of the overall infrastructure can be seen below:

graph LR;

input_metadata>Input metadata]
field_mapping>Field mapping]
output_file>Output file]
input_processor(Input processor)
metadata_entity(Metadata entity)
broker(Wrangler)
authenticator(Authenticator)
api(API)
output_processor(Output processor)

input_metadata-->|Provide metadata for archive|input_processor
field_mapping-->|Provide transformation rules for metadata fields|input_processor
metadata_entity-->|provide rules to generate and validate valid entities|input_processor
input_processor-->|Process metadata and be able to return a list of entities|api
authenticator-->|Provide token and REQUEST methods|api
api-->|"Provides with endpoints and ready [meta]data to"|broker
output_processor-->|"Provide entity-->record method"|broker
broker-->|Accessions and records saved|output_file

Examples can be found under the examples folder. WIP

Further documentation, including extended definitions and restrictions/requirements for all classes will be given below; for code-generated documentation, please refer to the docs.

How-to

Install

pip3 install biobroker

Run

This library does not support CLI access at the moment (Maybe in the future I could think of a CLI; although I would much rather have a simple GUI). As such, you will have to install and write scripts using the objects

Please see the examples folder.

Environment variables

• BROKER_LOG: Path to store the log for the execution of the library. Defaults to broker_log.txt if not set.
• API_ENVIRONMENT: Environment to be used by the API/Auth service.

Generate documentation

To generate documentation:

1. Go to the root of the repository: cd biobroker
2. cd docs
3. make clean html

This is usually not needed, as there is a GH action set-up to generate the documentation automatically. Use it only if you're not experienced with sphinx/unsure of how changes will look like in the RTD docs.

Adding a new class

If you add a new subclass, please add it under the autosummary directive in the RST file. You can see the examples in the existing docs/ folder.

Re-creating RST files from scratch

If the RST files need to be regenerated, it can be done as so:

sphinx-apidoc -Mf -o docs biobroker

This will regenerate the rst files with module content first! Just as I like it. However, we almost never want to regenerate the RST files - So please don't do this without opening an issue and a related PR.

Notes for developers

Regarding documentation:

• When adding development notes that are specific to a submodule, please add them under "README.md" within the submodule folder.

• When adding user guidelines or user-friendly descriptions for any of the submodules, please fill out the __init__ file within the submodule folder. User-friendly documentation for the library is generated from this file (For top/high-level module documentation) and from the code itself (for command/function documentation) using Sphinx

Regarding new entities:

• Read the Generic of each subclass you want to create. You will need to override some functions and properties!
• I don't know if this is the best practice, but I have a rule of thumb for making new functions:
  • If it's used by external entities (e.g. api object accessing a metadata_entity method), don't make it private
  • If it's just used by the entity internally (e.g. metadata_entity calling a function to process certain data), make it private.
• Add the new subclass under the 'init.py' document, both in __all__ and as an import