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LieberInstitute
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SPEAQeasy
SPEAQeasy: portable LIBD RNA-seq pipeline using Nextflow. Check http://research.libd.org/SPEAQeasy-example/ for an example on how to use this pipeline and analyze the resulting output files.
http://lieberinstitute.github.io/SPEAQeasy
MIT License
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transcript quantification (Salmon/Kallisto) should use trimmed reads
#110
gpertea
closed
10 months ago
1
improve storage usage, minimize duplication of FASTQ data
#109
gpertea
opened
12 months ago
2
Create a contribution guidelines documentation
#108
lcolladotor
opened
12 months ago
0
Adapt code to DSL2
#107
Nick-Eagles
closed
10 months ago
0
Add QC metrics generated by Monorail / recount3
#106
lcolladotor
opened
12 months ago
0
Add support for MultiQC and maybe other tools
#105
lcolladotor
opened
12 months ago
0
Translate course and intro documentation to Spanish
#104
lcolladotor
opened
12 months ago
0
Videos documenting how to install and run SPEAQeasy + describing outputs
#103
lcolladotor
opened
12 months ago
0
Develop a 1 day (max 2) workshop
#102
lcolladotor
opened
12 months ago
0
Guide for all setups that are atypical in some way
#101
lcolladotor
opened
12 months ago
0
[JHPCE] repeated fastqc failure on trimmed paired data
#100
gpertea
opened
1 year ago
15
BuildAnnotationObjects fails
#99
martinezvbs
closed
1 year ago
19
PullTranscriptFasta failing
#98
martinezvbs
closed
1 year ago
1
Improve or clarify what's supported for custom annotation
#97
Nick-Eagles
opened
1 year ago
0
Add support for more executors
#96
Nick-Eagles
opened
1 year ago
0
`BuildAnnotationObjects` halts with current version of R
#95
Nick-Eagles
closed
1 year ago
0
Construct `rse_tx` for rat
#94
Nick-Eagles
closed
1 year ago
5
InferStrandness failure, --force_strand not supported
#93
gpertea
closed
1 year ago
3
allow starting the workflow with existing read alignments (sorted BAM/CRAM files)
#92
gpertea
opened
1 year ago
3
Add support for running SPEAQeasy with one sample
#91
Nick-Eagles
opened
1 year ago
0
Use fixed version of nextflow
#90
Nick-Eagles
closed
2 years ago
0
Store run settings in output objects
#89
Nick-Eagles
closed
1 year ago
0
Gene end coordinates don't always agree with GTF
#88
Nick-Eagles
closed
1 year ago
2
Provide strandness-inference info in output objects
#87
Nick-Eagles
closed
1 year ago
1
Problematic use of caching for docker images
#86
Nick-Eagles
closed
2 years ago
0
regtools optimizations
#85
gpertea
closed
2 years ago
2
junction start coordinate bug
#84
gpertea
closed
2 years ago
1
Failure to build annotation objects for current version of R
#83
Nick-Eagles
closed
2 years ago
1
reprocess BrainSeq Phase 1 data
#82
gpertea
closed
2 years ago
11
Create Unit Tests
#81
Nick-Eagles
opened
2 years ago
0
Some metrics from FastQC are not documented
#80
Nick-Eagles
opened
3 years ago
0
R execution breaks on local installations with manually set working directories
#79
Nick-Eagles
closed
2 years ago
0
Verify the formatting and contents of `samples.manifest` in `PreprocessInputs`
#78
Nick-Eagles
closed
3 years ago
0
Choose whether alignment and/or pseudoalignment are run
#77
Nick-Eagles
opened
3 years ago
0
Rename exons with meaningful, unique IDs
#76
Nick-Eagles
opened
3 years ago
0
rse_tx: storing counts assay, not just tpm?
#75
gpertea
closed
3 years ago
0
"trimmed" and "bamFile" metrics are misleading
#74
Nick-Eagles
closed
3 years ago
0
Allocate reasonable memory for counts step on computing clusters
#73
Nick-Eagles
opened
3 years ago
1
Problems with package LieberInstitute/jaffelab
#72
ehatl
closed
3 years ago
6
Inconsistent compression among samples can break preprocessing step
#71
Nick-Eagles
closed
1 year ago
0
trim_mode "adaptive" failure
#70
gpertea
closed
3 years ago
0
Update to R 4.0
#69
Nick-Eagles
closed
3 years ago
1
Standardize code
#68
lcolladotor
closed
3 years ago
0
Make code more robust
#67
lcolladotor
closed
3 years ago
2
"How is geneMap$Length generated?" reveals more docs are needed
#66
lcolladotor
closed
3 years ago
0
Clean up output from generate_logs.sh
#65
Nick-Eagles
opened
3 years ago
1
Add support for passing arbitrary arguments to software
#64
Nick-Eagles
closed
3 years ago
0
Input samples are not necessarily mounted with docker
#63
Nick-Eagles
closed
4 years ago
0
biomaRt failure
#62
gpertea
closed
4 years ago
0
More robust testing across platforms needed with GHA
#61
Nick-Eagles
opened
4 years ago
0
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