search

Nextomics / NextDenovo

Fast and accurate de novo assembler for long reads
GNU General Public License v3.0
350 stars 52 forks source link

NextDenovo/bin/seq_dump nanopore.fastq does not exist! #105

Closed li0604 closed 3 years ago

li0604 commented 3 years ago

Hi, everybody. I am using the software named nextdenovo for my Nanopore sequencing data assembling. But there is an error and I don't how to solved it. The logfile was presented as following. Would you mind sharing your suggestion about this kind of error?

image

)RVV4FE(P8LWB 7G6{DK49D

moold commented 3 years ago
  1. It seems there is a space after file ...nanopore.fastq in input.fofn, so please delete it.
  2. you need set ava-ont for minimap2_options_raw and minimap2_options_cns.
  3. yourseed_cutoff is too short.

See here and here for more details.

li0604 commented 3 years ago

Thanks for your help and I corrected the reasons 2 and 3. The program is running.