ERROR：the input data is insufficient for an assembly

[Argonum-Clever2]

Error message *Suggested seed_cutoff (genome size: 680.00Mb, expected seed depth: 45, real seed depth: 9.80): 50001 bp [113064 ERROR] 2023-11-21 10:04:54 the input data is insufficient for an assembly.

Config file [General] job_type = local # local, slurm, sge, pbs, lsf job_prefix = nextDenovo task = all # all, correct, assemble rewrite = yes # yes/no deltmp = yes parallel_jobs = 20 # number of tasks used to run in parallel input_type = raw # raw, corrected read_type = ont # clr, ont, hifi input_fofn = ej.fofn workdir = /data0/stu_wangfang/software/NextDenovo

[correct_option] genome_size = 680m # estimated genome size sort_options = -m 20g -t 15 minimap2_options_raw = -t 8 pa_correction = 3 # number of corrected tasks used to run in parallel, each correct correction_options = -p 15

[assemble_option] minimap2_options_cns = -t 8 nextgraph_options = -a 1

Operating system LSB Version: :core-4.1-amd64:core-4.1-noarch Distributor ID: CentOS Description: CentOS Linux release 7.9.2009 (Core) Release: 7.9.2009 Codename: Core

GCC gcc version 4.8.5 20150623 (Red Hat 4.8.5-44) (GCC)

Python Python 3.9.12

[moold]

As the log says, the input data is insufficient for an assembly, so please input more data.

[Argonum-Clever2]

NextDenovo开发团队： 你好！       我输入了在你们公司测序得到的ONT数据，但是为什么他会显示数据不可用呢？您的意思是需要输入很多的数据吗？

Argonum @.***

 

------------------ 原始邮件 ------------------ 发件人: "Nextomics/NextDenovo" @.>; 发送时间: 2023年11月22日(星期三) 下午5:10 @.>; @.**@.>; 主题: Re: [Nextomics/NextDenovo] ERROR：the input data is insufficient for an assembly (Issue #195)

As the log says, the input data is insufficient for an assembly, so please input more data.

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[moold]

Yes, but if you want to get a more accurate answer, you have to follow here to provide more details.