The error of the seed_cutoff

[shehongbing]

Hi, when I used the seq_stat to analysis my data, but the results equal to 0 bp

*Suggested length cutoff of reads (genome size: 980000000, expected seed depth: 45) to be corrected: 0 bp

[moold]

Two possible reasons:

1. Total coverage < 45X, please sequencing more data.
2. Some reads do not have a corrected fasta/fastq format, please check your data format. This is a common issue for NanoPore data, such as, two records are collapsed to one line.

[shehongbing]

Thank you