version `GLIBC_2.14' not found

[unique379]

Hi I downloaded the package as suggested and i guess its already precomplied version as i was able to run NExtDenovo excutable and make was not present. But While running the pipeline GLIB errors found for numerous depended packages such as seq_dump: “/lib64/libc.so.6: version `GLIBC_2.14' not found (required by /softwares/NextDenovo/bin/seq_dump)”

Please provide me the source code or working recompiled version of the tool. i dont find in github as source have only doc zipped.

Thank Rupesh

[moold]

Pls provide more information

Operating system Which operating system and version are you using? You can use the command lsb_release -a to get it.

GCC What version of GCC are you using? You can use the command gcc -v to get it.

Python What version of Python are you using? You can use the command python --version to get it.

NextDenovo What version of NextDenovo are you using? You can use the command nextDenovo -v to get it.

[unique379]

Hi here are your answers:

OS:

LSB Version:    :base-4.0-amd64:base-4.0-noarch:core-4.0-amd64:core-4.0-noarch:graphics-4.0-amd64:graphics-4.0-noarch:printing-4.0-amd64:printing-4.0-noarch
Distributor ID: CentOS
Description:    CentOS release 6.7 (Final)
Release:    6.7
Codename:   Final

GCC: gcc version 4.4.7 20120313 (Red Hat 4.4.7-16) (GCC)

Python: Python 3.7.7

NextDenovo nextDenovo v2.3.0

ERRORS:

bin/seq_dump --help

bin/seq_dump: /lib64/libc.so.6: version `GLIBC_2.14' not found (required by bin/seq_dump)

bin/bam_sort --help

bin/bam_sort: error while loading shared libraries: liblzma.so.5: cannot open shared object file: No such file or directory

[moold]

Hi, your system and gcc are too old. I have recompile a version using gcc version 4.4.7, you can download NextDenovo.tar.gz and try again.

[unique379]

Thank you so much for providing the right precomplied version, its working for my system now. by the way, i ran stats (by following the tutorial; https://github.com/Nextomics/NextDenovo/blob/master/doc/TEST1.md) on my ONT fastq but tool suggested no seed ? what do you think about this situation ?

[``` Read length stat] Types Count (#) Length (bp) N10 400130 20662 N20 1058422 15036 N30 1889004 12464 N40 2874343 10612 N50 4028581 9052 N60 5393149 7564 N70 7065565 6001 N80 9264517 4358 N90 12421838 2914

Types Count (#) Bases (bp) Depth (X) Raw 21760220 115154951711 38.38 Filtered 3266343 2071372757 0.69 Clean 18493877 113083578954 37.69

*Suggested length cutoff of reads (genome size: 3000000000, expected seed depth: 45) to be corrected: 0 bp

[moold]

your data is not enough, change -d 45 to -d 30.