BELLA is a computationally efficient and highly accurate long-read to long-read aligner and overlapper. BELLA uses a k-mer based approach to detect overlaps between noisy, long reads. We demonstrated the feasibility of the k-mer based approach through a mathematical model based on Markov chains. BELLA provides a novel algorithm for pruning k-mers that are unlikely to be useful in overlap detection and whose presence would only incur unnecessary computational costs. Our reliable k-mers detection algorithm explicitly maximizes the probability of retaining k-mers that belong to unique regions of the genome. BELLA achieves fast overlapping without sketching using sparse matrix-matrix multiplication (SpGEMM), implemented utilizing high-performance software and libraries developed for this sparse matrix subroutine. Any novel sparse matrix format and multiplication algorithm would be applicable to overlap detection and enable continued performance improvements. We coupled BELLA's overlap detection with our newly developed vectorized seed-and-extend banded-alignment algorithm. The choice of the optimal k-mer seed occurs through our binning mechanism, where k-mer positions within a read pair are used to estimate the length of the overlap and to "bin" k-mers to form a consensus.We developed and implemented a new method to separate true alignments from false positives depending on the alignment score. This method demonstrates that the probability of false positives decreases exponentially as the overlap length between sequences increases.
These instructions will get you a copy of the project up and running on your local machine for development and testing purposes.
COMPILER: the software requires gcc-6 or higher with OpenMP to be compiled.
CUDA to compile and use GPU-accelerated pairwise alignment. You do not need CUDA to use CPU-based pairwise alignment. Our stand-alone GPU-based pairwise alignment, named LOGAN, can be found here.
Python3 and simplesam are required to generare the ground truth data. You can install simplesam via pip:
pip install simplesamClone the repository and enter it:
git clone https://github.com/giuliaguidi/bella
cd bellaBuild using makefile:
ln -s makefile-nersc Makefile
make bella (CPU-only) OR make bella-gpu (CPU/GPU)To run with default setting:
./bella -f <list-of-fastq> -o <output-name>BELLA requires a text file containing the path to the input fastq file(s) as the argument for the -f option. Example: input-example.txt
To show the usage:
./bella -hOptional flag description:
-f, --fastq arg List of Fastq(s) (required)
-o, --output arg Output Filename (required)
-k, --kmer arg K-mer Length (default: 17)
-x, --xdrop arg SeqAn X-Drop (default: 7)
-e, --error arg Error Rate (default: 0.15)
--estimate Estimate Error Rate from Data
--skip-alignment Overlap Only
-m, --memory arg Total RAM of the System in MB (default: 8000)
--score-deviation arg Deviation from the Mean Alignment Score [0,1]
(default: 0.1)
-b, --bin-size arg Bin Size for Binning Algorithm (default: 500)
--paf Output in PAF format
-g, --gpus arg GPUs Available (default: 1)
--split-count arg K-mer Counting Split Count (default: 1)
--hopc Use HOPC representation
-w, --window arg Window Size for Minimizer Selection (default: 0)
-s, --syncmer Enable Syncmer Selection
-u, --upper-freq arg K-mer Frequency Upper Bound (default: 8)
-l, --lower-freq arg K-mer Frequency Lower Bound (default: 2)
-h, --help UsageThe error rate is used to compute the adaptive alignment threshold. If using PacBio CCS/HiFi please set --error 0.005.
The parallelism during the overlap detection phase depends on the available number of threads and on the available RAM [Default: 8000MB].
Use -DOSX or -DLINUX at compile time to estimate available RAM from your machine. If your machine has more RAM than the default one, using -DOSX or -DLINUX would make the ovelap detection phase faster.
BELLA outputs alignments in a format similar to BLASR's M4 format. Example output (tab-delimited):
[A ID] [B ID] [# shared k-mers] [alignment score] [overlap length] [n=B fwd, c=B rc] [A start] [A end] [A length] [B start] [B end] [B length]The positions are zero-based and are based on the forward strand, whatever which strand the sequence is mapped. If -p option is used, BELLA outputs alignments in PAF format. Example output (tab-delimited):
[A ID] [A length] [A start] [A end] ["+" = B fwd, "-" = B rc] [B ID] [B length] [B start] [B end] [alignment score] [overlap length] [mapping quality]The repository contains also the code to get the recall/precision of BELLA and other long-read aligners (Minimap, Minimap2, DALIGNER, MHAP and BLASR).
minimap2 -ax map-pb ref.fa pacbio-reads.fq > aln.sam # for PacBio subreads
samtools view -h -Sq 10 -F 4 aln.sam > mapped_q10.sam # remove reads with quality values smaller than 10
samtools view -h mapped_q10.sam | grep -v -e 'XA:Z:' -e 'SA:Z:' | samtools view -S -h > unique_mapped_q10.sam # remove reads mapped to multiple locations
python3 SAMparser.py <bwamem/minimap2-output>python scripr/mafconvert.py axt <maf-file> > <ground-truth.txt>To run the evaluation program:
cd benchmark
make result./result -G <grouth-truth-file> [-B <bella-output>] [-m <minimap/minimap2-output>] [-D <daligner-output>] [-L <blasr-output>] [-H <mhap-output>] [-M <mecat-output>] [-i <mecat-idx2read-file>]If the output of BELLA is in PAF format, you should run it using minimap2 -m flag.
To show the usage:
./result -hNOTE: add -z flag if simulated data is used.
You can download an E. coli 30X dataset here to test BELLA. For this dataset, you can use the following single mapped ground truth to run the evaluation code: ecsample_singlemapped_q10.txt. A detailed description of the procedure we use to generate the ground truth for real data can be found in our preprint.
You can run the evaluation code located in /bench folder as:
./result -G ecsample_singlemapped_q10.txt -B <bella-output>
Error rate estimation might have gone wrong. If the error estimated is greater than 1, the adaptive alignment threshold would be so high that no alignments would pass the threshold. Please check if your fastq file has proper quality values. If not, please define an error rate using command line options.
To cite our work or to know more about our methods, please refer to:
BELLA: Berkeley Efficient Long-Read to Long-Read Aligner and Overlapper. Giulia Guidi, Marquita Ellis, Daniel Rokhsar, Katherine Yelick, Aydın Buluç. bioRxiv 464420; doi: https://doi.org/10.1101/464420.
Berkeley Efficient Long-Read to Long-Read Aligner and Overlapper (BELLA), Copyright (c) 2018, The Regents of the University of California, through Lawrence Berkeley National Laboratory (subject to receipt of any required approvals from the U.S. Dept. of Energy) Giulia Guidi and Marco Santambrogio. All rights reserved.
If you have questions about your rights to use or distribute this software, please contact Berkeley Lab's Innovation & Partnerships Office at IPO@lbl.gov.
NOTICE. This Software was developed under funding from the U.S. Department of Energy and the U.S. Government consequently retains certain rights. As such, the U.S. Government has been granted for itself and others acting on its behalf a paid-up, nonexclusive, irrevocable, worldwide license in the Software to reproduce, distribute copies to the public, prepare derivative works, and perform publicly and display publicly, and to permit other to do so.
Funding provided in part by DOE ASCR through the Exascale Computing Project, and computing provided by NERSC. Thanks to Rob Egan and Steven Hofmeyr for valuable discussions. Thanks to NECST Laboratory and Ed Younis for key collaborations.