To install the dependencies and create the environment:
. marple-install.sh
This script also adds the functions to run the pipeline in
.bashrc
.
To concatenate and transfer the fastq files created in MinKnow for pgt or pst:
transfer-pgt <name of experiment> <barcodeXX>=<name of sample> <barcodeYY>=<name of sample> ...
transfer-pst <name of experiment> <barcodeXX>=<name of sample> <barcodeYY>=<name of sample> ...
To run MARPLE:
marple
These functions can be executed from anywhere in the system.
[!IMPORTANT] The functions here, assume the reads are in
/var/lib/minknow/data/
and marple in/home/$USER/
.