Closed S-111 closed 5 months ago
I think that if raw reads are used for scaffolding, the results may be not much different than if only valid read are used. So, I think that it is unnecessary to extract and use only valid reads for scaffolding.
Thank you
I understand. Thank you very much for your answer!
I assembled a genome using hifiasm's Hi-C mode with HiFi reads and Hi-C reads. The Hi-C reads used were raw data. In this case, before running GreenHill, should I map the Hi-C reads to the assembled genome, extract valid reads, and then use only the valid reads for scaffolding?