ShunOuchi / GreenHill

De novo chromosome-level scaffolding and phasing tool using Hi-C
GNU General Public License v3.0
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Preprocessing of Hi-C reads #34

Closed S-111 closed 5 months ago

S-111 commented 6 months ago

I assembled a genome using hifiasm's Hi-C mode with HiFi reads and Hi-C reads. The Hi-C reads used were raw data. In this case, before running GreenHill, should I map the Hi-C reads to the assembled genome, extract valid reads, and then use only the valid reads for scaffolding?

ShunOuchi commented 5 months ago

I think that if raw reads are used for scaffolding, the results may be not much different than if only valid read are used. So, I think that it is unnecessary to extract and use only valid reads for scaffolding.

Thank you

S-111 commented 5 months ago

I understand. Thank you very much for your answer!