TheJacksonLaboratory / splicing-pipelines-nf

Repository for the Anczukow-Lab splicing pipeline
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Check for strandedness #225

Closed angarb closed 3 years ago

angarb commented 3 years ago

Problem

Currently, we are not using a STAR parameter that will allow us to check the strandedness of our data.

Solution

Add parameter -quantmode Genecounts to STAR Pull this generated ReadsPerGene.out.tab file to the results folder.