pyroptosis_signature_data_analysis
Analysis Repository for the Interstellar project. For image analysis and image-based profiling for this project, please see pyroptosis_signature_image_profiling
Workflow:
Cells were incubated for 1h in the inhibitors, and then incubated for 6h in each inducer. For more information on plate layout and experimental design please see pyroptosis_signature_image_profiling.
Treatments of Interest (All in this table are with DMSO 0.025% inhibitor)
| Inducer | Number of Doses | Dose Concentration(s) | Function |
|---|---|---|---|
| DMSO | 1 | 0.025% | Vehicle Control |
| Thapsigargin | 2 | 1uM 10uM |
ER Stress Induced Apoptosis |
| H202 | 2 | 100nM 100uM |
Cell Death |
| Flagellin | 2 | 0.1ug/mL 1.0ug/mL |
Induce Pyroptosis |
| LPS | 3 | 0.01ug/mL 1.0ug/mL 100ug/mL |
Induce Pyroptosis |
| LPS + Nigericin | 1 * 3 = (3) | 1ug/mL + 1uM 1ug/mL + 3uM 1ug/mL + 10uM |
Induce Pyroptosis |
Other Treatments and combinations
| Inducers | # of Doses | Dose Concentration(s) | Inhibitors | # of Doses | Dose Concentration(s) | Function |
|---|---|---|---|---|---|---|
| Media | 1 | 0 | Media | 1 | 0 | Control |
| DMSO | 1 | 0.0250% | DMSO | 2 | 0.025% 1.0% |
Vehicle Control |
| DMSO | 1 | 0.0250% | Z-VAD-FMK | 2 | 30uM 100 uM |
Inhibit Inflamasome |
| Thapsigargin | 2 | 1uM 10uM |
DMSO | 1 | 0.025% | ER Stress Induced Apoptosis |
| H202 | 2 | 100nM 100uM |
DMSO | 1 | 0.025% | Cell Death |
| H202 | 2 | 100nM 100uM |
Z-VAD-FMK | 1 | 100uM | Inhibit Inflamasome |
| H202 | 2 | 100nM 100uM |
Disulfiram | 1 | 1uM | Cell Death |
| Flagellin | 2 | 0.1ug/mL 1ug/mL |
DMSO | 1 | 0.025% | Induce Pyroptosis |
| Flagellin | 2 | 0.1ug/mL 1ug/mL |
Disulfiram | 1 | 1uM | Induce Pyroptosis Inhibit Inflamasome |
| LPS | 5 | 0.01ug/mL 0.1ug/mL 1.0ug/mL 10.0ug/mL 100.0 ug/mL |
DMSO | 1 | 0.025% | Induce Pyroptosis |
| LPS | 1 | 10.0ug/mL | Z-VAD-FMK | 1 | 100uM | Induce Pyroptosis / Inhibit Inflamasome |
| LPS | 1 | 10.0ug/mL | Disulfiram | 3 | 0.1uM 1uM 2.5uM |
Induce Pyroptosis / Inhibit Inflamasome |
| LPS + Nigercin | 2 * 3 = (6) | 1ug/mL+1ug/mL 1ug/mL+3ug/mL 1ug/mL+10ug/mL 100ug/mL+1ug/mL 100ug/mL+3ug/mL 100ug/mL+10ug/mL |
DMSO | 1 | 0.025% | Induce Pyroptosis |
| LPS + Nigercin | 1 * 1 = (1) | 1ug/mL+10ug/mL | Z-VAD-FMK | 1 | 100uM | Induce Pyroptosis Inhibit Inflamasome |
| LPS + Nigercin | 1 * 1 = (1) | 1ug/mL+10ug/mL | Disulfiram | 1 | 1uM | Induce Pyroptosis Inhibit Inflamasome |
| Disulfiram | 3 | 0.1uM 1uM 2.5uM |
DMSO | 1 | 0.025% | Pyroptosis Inhibitor |