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WeisongZhao / Sparse-SIM

Official MATLAB implementation of the "Sparse deconvolution" -v1.0.3
https://weisongzhao.github.io/Sparse-SIM/
Open Data Commons Open Database License v1.0
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What is "effective NA" in the software #13

Open GalaxyNight-day opened 1 year ago

GalaxyNight-day commented 1 year ago

First of all, congratz on new paper (SACD)! I tried it, and it works really well :)

Question: what is "effective NA" in the sparse-sim software? if I were to use confocal with 1.27NA water lens, for example, what value should I put in...?

Thank you very much!

WeisongZhao commented 1 year ago

Hi, Thanks a lot for your kind words :).

The effective NA represents the effective resolution of a system (wavelength/2NA), and it is adjustable in a reasonable range. For example of SIM, we usually use 3 as effective NA for 1.7 (objective NA) + 1.3 (illumination NA).

If you are using a confocal microscope without a super-resolution module, you can use the objective NA directly.