Closed arish-n-shah closed 3 years ago
A couple questions:
qsub
SLURM-compatible? Is qsub
executed directly on your computer, or do you SSH into an HPC cluster first?xpresspipe test
and xpresspipe riboseq`, so I am wondering if there was an internet connection issue? Have you tried rerunning the script to see if the connection issue resolves itself?alignment.sh
script to source activate xpresspipe
and see if that does anything?
When trying to use xpresspipe to align riboseq data, there seems to be a problem in urllib. MacOS Big Sur 11.2 M1 Chip
I have an xpresspipe environment activated and it tests correctly:
Now, I qsub my Alignment.sh file:
"processed" is a folder which contains split, UMI-deduplicated-and-removed, adapter-removed, footprint-seq and RNA-seq fastq files "riboseq_output" is an empty folder in the current dir "LSU_IP" is just the name of the experiment "Danio_rerio.GRCz11.cdna.all.fa" is the all cDNA file downloaded from Ensembl "riboseq_reference/genome_fasta" is a folder with 25 chromosome.fa files "riboseq_reference/..._CT.gtf" is the gtf file created after using curateReference
The pipeline throws this error:
Keep this to auto-ping a maintainer @j-berg