As we have 2 replicates of control and 2 replicates of treated group :
cat simple_counts.txt | Rscript deseq1.r 2x2 > results_deseq1.tsv
error: results_deseq1.tsv is 0 bytes
the error
Attaching package: ‘BiocGenerics’
The following objects are masked from ‘package:parallel’:
Loading required package: Biobase
Welcome to Bioconductor
Vignettes contain introductory material; view with
'browseVignettes()'. To cite Bioconductor, see
'citation("Biobase")', and for packages 'citation("pkgname")'.
Loading required package: locfit
locfit 1.5-9.1 2013-03-22
Loading required package: lattice
Welcome to 'DESeq'. For improved performance, usability and
functionality, please consider migrating to 'DESeq2'.
Error in newCountDataSet(counts, conditions) :
length(conditions) == ncol(countData) is not TRUE
Calls: newCountDataSet -> stopifnot
Execution halted
As we have 2 replicates of control and 2 replicates of treated group : cat simple_counts.txt | Rscript deseq1.r 2x2 > results_deseq1.tsv error: results_deseq1.tsv is 0 bytes the error Attaching package: ‘BiocGenerics’
The following objects are masked from ‘package:parallel’:
The following objects are masked from ‘package:stats’:
The following objects are masked from ‘package:base’:
Loading required package: Biobase Welcome to Bioconductor
Loading required package: locfit locfit 1.5-9.1 2013-03-22 Loading required package: lattice Welcome to 'DESeq'. For improved performance, usability and functionality, please consider migrating to 'DESeq2'. Error in newCountDataSet(counts, conditions) : length(conditions) == ncol(countData) is not TRUE Calls: newCountDataSet -> stopifnot Execution halted