bdaisley / isolateR

Automated processing of Sanger sequencing data, taxonomic profiling, and generation of microbial strain libraries
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isoQC parameter 'keep_old_reps=TRUE' fails in rare cases when merging new + old isoLIB files #12

Closed bdaisley closed 3 months ago

bdaisley commented 3 months ago

Description: The isoLIB parameter 'keep_old_reps=TRUE' should always prevent overwriting of Library 1 (old library) sequence grouping names when merging with Library 2 (new library). However, in some cases Library 1 sequence groups are not prioritized. This issue likely stems from misordering of VSEARCH --uc output file due to multithreading.

bdaisley commented 3 months ago

Fixed in 440701e