Just in case anybody else needs such a thing!
Example usage, to interface with CLR components:
### requirements.txt
pythonnet
### your_app.py
import clr
from System.Collections.Generic import Dictionary
from System import String, Int32, Type
dict1 = Dictionary[String, String]()
dict2 = Dictionary[String, Int32]()
dict3 = Dictionary[String, Type]()
### Dockerfile
FROM benley/wine-python-dotnet
EXPOSE 8080
RUN python your_app.py
Normal Python stuff should generally just work. Compiling Python extensions may require adding more components to the wine environment, but I'm pretty sure it can be done.