Closed hmzawz2 closed 5 months ago
Hi Hmzawz2,we used the program EMAN2 to calculate the FSC,the specific command we used is: e2proc3d.py model1 --calcfsc=model2 fsc.txt. You can replace the model1 and model2 with your file, and all cross-correlation values are contained in the fsc.txt. Don't hesitate to contact us if you have further problems.
Thanks, I will do more test.
Hello, recently I have been reproducing your work based on the EPFL dataset. For calculating the resolution of reconstruction results, I used the code from repository to calculate (parameters are sz=512, psz=5nm zm=1). Under this set of parameters, the calculated resolution of IsoVEM is 27.9nm. Could you please share your calculating code of FSC and parameters were used to calculate the FSC resolution in the IsoVEM paper? Thank you in advance!