dan-landau / IronThrone-GoT

processes GoT amplicon data and generates a table of metrics
26 stars 6 forks source link

running circle GoT #1

Closed gitske closed 4 years ago

gitske commented 4 years ago

Congratulations to the Landau-lab team for this well-made script!

While the linear example reads of R1 consist of barcode and UMI (length 26) the circular example reads have a length of 151 (the same as the corresponding R2 reads). What, besides barcode + UMI, is in these circular R1 reads? Is the length necessary? Thanks in advance!