digitalcytometry / ecotyper

EcoTyper is a machine learning framework for large-scale identification of cell states and cellular ecosystems from gene expression data.
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Question about recovery scRNA in ecotyper #85

Closed LynnChan99 closed 10 months ago

LynnChan99 commented 10 months ago

Sorry for bothering you again. I am running ecotyper to train my scRNA data, and some problems bothers me now.

For public or our own scRNA data, they don't have or have annotated celltype. For example, if I want to run Tutorial 2 in GEO cohort, and It has well-annotated celltype, but the major celltype, nor the minor celltype, can't be divided into the celltype presented in your example data exactly. It has celltype like "PVL", "cycling T-cells", and so on, thus making me confused about preparing the annotation files. Should I re-annotate the celltype? But I also found nowhere mentioned about celltype annotation.

I am so appreciated if I could get your help in preparing the annotation file of recoverying scRNA data step.

THANK YOU SO MUCH!!!