Open nayanvs opened 1 year ago
Hello @NayanVS. The classify
function of MLSeq offers several normalization and transformation alternatives, as well as another option to use them untouched which is your case. The argument "normalize" has an option "none"; however, it is not included in the "preProcess" argument, which should be included obviously. I will check the source codes for this issue and fix it.
Thank you, Best.
Hello Prof. @dncR, thank you for your feedback. I was able to run the chunk by replacing the preProcessing argument with the normalize="none" argument. I was wondering how appropriate is this.
Also, I have found that your algorithm is equally effective in other sequencing-based classification problems of biological samples and is not limited to expression counts. But I think you are aware of that since you have mentioned CHIP-seq etc in vignette.
Why normalization by the following is required when I already include normalized and batch effect-adjusted data for the training? deseq-vst deseq-rlog deseq-logcpm tmm-logcpm