instead of doing presence/absence of sizes, it would be useful to find primers in all the genomes but where the ingroup is distinguishable using sequencing of the amplicon.
primers in both in- and out-groups
amplicons are "different enough" between in- and out-groups
instead of doing presence/absence of sizes, it would be useful to find primers in all the genomes but where the ingroup is distinguishable using sequencing of the amplicon.