egaffo / circompara2

Improved bioinformatic pipeline to identify and quantify circRNA expression from RNA-seq data by combining multiple circRNA detection methods
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I am having trouble generating the back_spliced_junction.bed file during the CIRCexplorer2_tophat fusion step while using the circompara2 docker. #24

Closed bioxliuze closed 6 months ago

bioxliuze commented 6 months ago

@egaffo Hello, I am having trouble generating the back_spliced_junction.bed file during the CIRCexplorer2_tophat fusion step while using the circompara2 docker.I have 12 samples, and 11 of them run smoothly, only the one named H7 sample fails to run.

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This is a sample that runs normally.

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This is the sample H7

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I don’t know why only the H7 sample would produce an error.

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There seems to be plenty of memory space left.

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I split the H7 sample into two parts.

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Interestingly, H701 can run normally while H702 encountered an error.

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After converting the BAM file of H7 into fastq, the entire process can be successfully completed.But this step will lose some information.

I hope to receive your help, thank you!