A nextflow pipeline with a GMS touch for running the ARTIC network's fieldbioinformatics tools (https://github.com/artic-network/fieldbioinformatics).
Find DAG and other figures here
To test the pipeline, an example dataset for both Illumina and Nanopore (nanopolish, medaka) datafiles (from ConnerLab) provided.
--scheme nCoV-2019/V3/
--scheme midnight-primers/V1/
--scheme eden-primers/V1/
To run the artic pipeline, please change the nanopore.config 'min_length' (default = 400) and 'max_length' (default = 700)
For more parameters setup, please see the ConnerLab documentation
nextflow run main.nf -profile singularity \
--illumina --prefix "test_illumina" \
--directory .github/data/fastqs/ \
--outdir illumina_test
nextflow run main.nf -profile singularity \
--nanopolish --prefix "test_nanopore_nanopolish" \
--basecalled_fastq .github/data/nanopore/20200311_1427_X1_FAK72834_a3787181/fastq_pass/ \
--fast5_pass .github/data/nanopore/20200311_1427_X1_FAK72834_a3787181/fast5_pass/ \
--sequencing_summary .github/data/nanopore/20200311_1427_X1_FAK72834_a3787181/sequencing_summary_FAK72834_298b7829.txt \
--outdir nanopore_nanopolish
nextflow run main.nf -profile singularity \
--medaka --prefix "test_nanopore_medaka" \
--basecalled_fastq .github/data/nanopore/20200311_1427_X1_FAK72834_a3787181/fastq_pass/ \
--outdir nanopore_medaka
sample_name
|___ fast5_pass/
|___ fastq_pass/
|___ sequencing_summary.txt
nextflow run main.nf -profile singularity,sge \
--nanopolish --prefix "test_nanopore" \
--basecalled_fastq .github/data/nanopore/20200311_1427_X1_FAK72834_a3787181/fastq_pass/ \
--fast5_pass .github/data/nanopore/20200311_1427_X1_FAK72834_a3787181/fast5_pass/ \
--sequencing_summary .github/data/nanopore/20200311_1427_X1_FAK72834_a3787181/sequencing_summary_FAK72834_298b7829.txt \
--outdir nanopore_test
nextflow run main.nf -profile singularity,sge \
--medaka --prefix "test_nanopore_medaka" \
--basecalled_fastq .github/data/nanopore/20200311_1427_X1_FAK72834_a3787181/fastq_pass/ \
--outdir nanopore_medaka
sample_name
|___ fastq/
nextflow run main.nf -profile singularity,sge \
--illumina --prefix "test_illumina" \
--directory .github/data/fastqs/ \
--outdir illumina_test
1.To update your container image to the latest version from dockerhub, please delete your local image first before running the analysis pipeline.