search

hartleys / JunctionSeq

The JunctionSeq R package is a powerful tool for testing and visualizing differential usage of exons and splice junctions in next-generation, high-throughput RNA-Seq experiments.
28 stars 15 forks source link

Error: all samples have 0 counts for all genes. check the counting script #51

Open HeenaBioinfo opened 4 years ago

HeenaBioinfo commented 4 years ago

Using single-core execution. getAllJunctionSeqCountVectors: dim(counts) = 809447,6 (Fri Jul 31 16:54:53 2020) getAllJunctionSeqCountVectors: dim(gct) = 54727,6 getAllJunctionSeqCountVectors: out generated. dim = 809447,12 (Fri Jul 31 16:55:18 2020) -----> count vectors generated (Fri Jul 31 16:55:18 2020) -----> generating DESeqDataSet... (Fri Jul 31 16:55:18 2020) Error in DESeqDataSet(se, design = design, ignoreRank) : all samples have 0 counts for all genes. check the counting script.

Please help regarding this error