Option 1 - try to describe the species with 5 subsamples from each and finish the rest of the available forereef and backreef

Backreef has 11 months POC-466 - All Months Available - 1 tissue piece per month - backreef sample not yet analyzed (11) POC-467 - All Months Available - 5 tissue piece per month - backreef sample not yet analyzed - will be used to describe Pgrandis (55) POC-468 - All Months Available - 1 tissue piece per month - backreef sample not yet analyzed (11) POC-469 - All Months Available - 1 tissue piece per month - backreef sample not yet analyzed (11) POC-470 - All Months Available - 1 tissue piece per month - backreef sample not yet analyzed (11)

POC-471 - All Months Available - 4 tissue piece per month - will be used to describe P. tuahiniensis - can already see data in some months from first slides (44) POC-472 - All Months Available - 4 tissue piece per month - will be used to describe P. verrucosa - can already see data in some months from first slides (44) POC-474 - All Months Available - 4 tissue piece per month - will be used to describe P. c.f. effusa - can already see data in some months from first slides (44) POC-475 - All Months Available - 4 tissue piece per month - will be used to describe P. meandrina - can't see much from first slides in any months (44)

total backreef = 275

Forereef has 10 months POC-476 - All Months Available - 1 tissue piece per month (10) POC-482 - All Months Available - 1 tissue piece per month (10) POC-483 - All Months Available - 1 tissue piece per month (10) POC-484 - All Months Available - 1 tissue piece per month (10) POC-485 - All Months Available - 1 tissue piece per month (10)

total forereef = 50

total samples = 325

Option 2 - use 2024 to describe the species and just do a balanced sample size for backreef to finish this paper

POC-466 - 1 tissue piece per month - backreef sample not yet analyzed (11) - P. meandrina POC-467 - 1 tissue piece per month - backreef sample not yet analyzed (11) - P. meandrina POC-468 - 1 tissue piece per month - backreef sample not yet analyzed (11) - P. grandis POC-469 - 1 tissue piece per month - backreef sample not yet analyzed (11) - P. meandrina POC-470 - 1 tissue piece per month - backreef sample not yet analyzed (11) - P. tuahiniensis

This would result in n=3 P. tuahiniensis and n=3 P. meandrina available for the backreef

total samples = 55

hputnam commented 3 months ago

@chloe-gilligan @daniellembecker can you please organize, prepare, and send the following to NOVA?

POC-466 - 1 tissue piece per month - backreef sample not yet analyzed (11) - P. meandrina POC-467 - 1 tissue piece per month - backreef sample not yet analyzed (11) - P. meandrina POC-468 - 1 tissue piece per month - backreef sample not yet analyzed (11) - P. grandis POC-469 - 1 tissue piece per month - backreef sample not yet analyzed (11) - P. meandrina POC-470 - 1 tissue piece per month - backreef sample not yet analyzed (11) - P. tuahiniensis

chloe-gilligan commented 3 months ago

@hputnam @daniellembecker samples started decalcifying 20240603 with 10% HCl, I switched out the HCl twice since then and today increased concentration to 20% HCl. I have emailed NOVA and waiting for a response

chloe-gilligan commented 2 months ago

Samples were sent Tuesday 20240618 Below are the instructions sent to NOVA as well as a list of samples mailed to be processed into histology slides Histology sample information

hputnam / Moorea_Pocillopora

Submit Additional POC Histology to NOVA #9

Option 1 - try to describe the species with 5 subsamples from each and finish the rest of the available forereef and backreef

Option 2 - use 2024 to describe the species and just do a balanced sample size for backreef to finish this paper